Fig. 6.

Comparison of the effect of 2 h (checkered bars) and 20 h (solid bars) resting periods for cells after thawing, before adding to the assay. D1–4 CEF/CMV refer to the specific donor and peptide pool tested. Background reactivity for all donors and testing conditions was between 0 and 5 spots (not shown). The standard error is shown. * Indicates a statistical significant difference of P < 0.01 in spot counts between 2 and 20 h resting periods for a given donor and reagent; # indicates in statistical difference with P < 0.05 (Student’s t test)