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. 2000 Dec 25;151(7):1353–1368. doi: 10.1083/jcb.151.7.1353

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Distribution of C1δ-YFP and PLCδ-PH-CFP during phagocytosis. (A–C) Representative cells expressing C1δ-GFP or C1δ-YFP immediately before (A) and 10 min after addition of 10 μM ionomycin (B), or after treatment with 200 nM TPA for 10 min at 15°C (C). (D) Confocal fluorescence images of RAW cells coexpressing C1δ-YFP (yellow) and PLCδ-PH-CFP (blue) undergoing phagocytosis of RBCs. Subscripts (e.g., D₁) refer to the time in minutes at which the fluorescence images were captured. D₁, D_3.5, D₆, and D_7.5 show overlays of the PLCδ-PH-CFP and C1δ-YFP signals. The insets show separately the fluorescence of PLCδ-PH-CFP (labeled C) and C1δ-YFP (labeled Y) of the areas boxed by the dotted line. In D₁ and D_3.5, three formed phagosomes are indicated with filled white circles. Bars, 10 μm.