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. 2000 Dec 25;151(7):1591–1598. doi: 10.1083/jcb.151.7.1591

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Human sperm H2B is a variant histone capable of forming complexes with telomeric DNA in vitro. (a) Separation of the purified sperm and somatic (HeLa) histone fractions H2B and H2A in Triton/acetic acid/urea PAGE. The arrow shows a sperm-specific variant of H2B. (b) 3 μg of crude hSTBP_TR, 50 ng of HeLa H2B, or 50 ng of sperm H2B were incubated with 0.5 ng of labeled [TTAGGG]₁₂ in binding buffer containing 100 μg/ml BSA and 100 ng fragmented E. coli DNA and separated in 6% PAGE. SpH2B-telomere DNA complex is shown by the arrow.