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. 2000 Dec 25;151(7):1537–1548. doi: 10.1083/jcb.151.7.1537

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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In situ mRNA determination of cDNAs coding for NPY and GAD. Thionin staining (Thionin) of a cross-section through the cortex with layers I–VI is shown, as indicated for wt (top) or synRas-TG (bottom) mice. Bar, 150 μm. The density and intensity of NPY mRNA–expressing neurons is upregulated throughout the cortex of synRas-TG mice (NPY, top and bottom). Bar, 100 μm. However, GAD cDNA expression is not affected (GAD, top and bottom). Bar, 100 μm. Note the increased cross-sectional length of the total cortical layer from the pia mater to the borderline layer VI between wt (∼720 μm) and synRas-TG mice (compare stars).