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. 2000 Dec 25;151(7):1381–1390. doi: 10.1083/jcb.151.7.1381

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Specificity of 53BP1-reactive monoclonal antibodies (MAb). Nuclear extracts of nontransfected cells and whole cell extracts from cells stably expressing HA-tagged full-length or truncated 53BP1 proteins were immunoblotted (IB) with a 53BP1-reactive monoclonal antibody (clone WI1) at a 1:20 dilution or an HA tag–reactive polyclonal antibody (PAb; clone Y11) used at a 1:1,000 dilution. Endog., endogenous.