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. 2000 Dec 25;151(7):1561–1574. doi: 10.1083/jcb.151.7.1561

Figure 2.

Figure 2

Coilin localization in parental HeLa and HeLaGFP-coilin cells. Micrographs show both differential interference contrast (DIC) and fluorescence images of the HeLaGFP-coilin cells (A–C) and the parental HeLa cells (D–F). Representative optics sections from deconvolved data sets of the HeLaGFP-coilin cells are presented (B and C) with GFP-coilin in green (B), and counterstaining detecting both endogenous p80-coilin and GFP-coilin in red (C). (A) The DIC image of the same field of cells shown in B and C. Representative optical sections from deconvolved data sets from the parental HeLa cell line are presented (E and F) with endogenous p80 coilin detected with anticoilin antibody in red (F). No GFP fluorescence is detected in the parental HeLa cell line (E). (D) The DIC image of the same field of cells shown in F. For both the parental and HeLaGFP-coilin cells, endogenous p80 coilin and GFP-coilin show an identical pattern, consisting of a diffuse nucleoplasmic signal together with several bright foci of different sizes. Bar, 5 μm.