Figure 6.
In vivo time-lapse three-dimensional analysis of an HeLaGFP-coilin cell nucleus showing movement of CBs through the nucleoplasm and separation of mini-CBs from Cajal bodies. 12 three-dimensional time-lapse images out of a total of 22 are shown as a montage. The image at each time point is a maximum intensity projection of 20 optical sections (0.5 μm each) scanning the entire cell nucleus. Data were collected every 3 min over a period of 2.25 h. Note the movement of a CB (magenta arrowheads) through the nucleoplasm from right to left bringing it into close proximity to a Cajal body that was located at the opposite side of the nucleus at time 0.00. A mini-CB (blue arrowhead) can be seen separating from a larger Cajal body located at the nuclear periphery, moving to the nucleolus and returning back to the same Cajal body from where it originated. An animated time-lapse version of this process is available at http://www.jcb.org/cgi/content/full/151/7/1561/DC1. Bar, 15 μm.