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. 2000 Dec 25;151(7):1483–1500. doi: 10.1083/jcb.151.7.1483

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Flightin null assembles long myofilaments and sarcomeres during pupal development. EMs of thin longitudinal sections of IFM from wild-type (left) and fln⁰ (right) late stage pupa. At this developmental stage, wild-type sarcomeres show an average length of 3.1 μm, while fln⁰ sarcomeres show an average length of 3.8 μm and are narrower. Note the presence of two transverse electron dense stripes flanking the M line (arrows). These stripes are reminiscent of the transient, particle-dense stripes that have been observed in some late pupa of wild-type IFM (Reedy and Beall 1993). In normal flies, the double band is present for a few hours just before the end of pupation; in fln⁰ flies, the double band sometimes persists into adulthood.