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. 2000 Sep 18;150(6):1299–1310. doi: 10.1083/jcb.150.6.1299

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The WH1 domain is not required for the ability of PI(4,5)P₂ to activate N-WASP. (A) A 10% SDS–polyacrylamide gel showing purified protein preparations of GST-tagged wild-type N-WASP (GST-N-WASP) or a GST-tagged truncation mutant of N-WASP lacking the WH1 domain (GST-(ΔWH1) N-WASP). (B) Actin polymerization (1.3 μM actin, 30 nM Arp2/3 complex) in the presence of the indicated combinations of GST-N-WASP (50 nM), GST-(ΔWH1)N-WASP (50 nM), Cdc42-GTPγS (100 nM), or PI(4,5)P₂-containing vesicles (1 μM).