Table 2.
Pi Inhibition constants (I0.5) of R4, R20, and R32 genetic revertants and the WT recombinant, and P52L enzymes
| 3-PGA, mM | wt | P52L | R4 | R20 I0.5, mM | R32 | Up-Reg-1 |
|---|---|---|---|---|---|---|
| 0.025 | n.d. | n.d. | n.d. | n.d. | n.d. | 0.6 (0.2) |
| 0.10 | n.d. | 0.68 | n.d. | n.d. | n.d. | n.d. |
| 0.125 | n.d. | n.d. | n.d. | 0.21 (.04) | n.d. | 3.1 (0.1) |
| 0.25 | 0.07 | n.d. | 1.20 (.35) | 0.57 (.12) | 0.32 (.11) | 4.7 (1.0) |
| 0.50 | 0.15 | n.d. | 1.38 (.25) | 0.70 (.28) | 0.58 (.11) | n.d. |
| 2.5 | n.d. | 0.84 | n.d. | n.d. | n.d. | n.d. |
| 3.00 | 0.63 | n.d. | 13.0 (4.0) | n.d. | 1.04 (.40) | n.d. |
| 4.5 | n.d. | 2.5 | n.d. | n.d. | n.d. | n.d. |
Values (in mM) are the average of at least two assays. n.d., not determined.