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. 2000 Sep 18;150(6):1335–1348. doi: 10.1083/jcb.150.6.1335

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Asymmetric activation of zebrafish p38 during the early cleavage period. Top view of zebrafish embryos after fluorescent immunostaining with the anti-p38 antibody at the 2-cell (A), 4-cell (D), 8-cell stage (G), and the 16-cell stage (J), or with anti–dp-p38 antibody (anti–ACTIVE p38™ antibody) at the 2-cell (B and C), 4-cell (E and F), 8-cell (H and I), and the 16-cell stage (K and L). The distribution of p38 or activated p38 (red) was determined using a confocal microscope. Images were taken at a depth of 100 μm from the animal pole surface. Bar, 100 μm.