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. 2000 Sep 18;150(6):1335–1348. doi: 10.1083/jcb.150.6.1335

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Impaired symmetric and synchronous cleavage by DN-p38. (A–D) Phenotypes after microinjection of the WT-p38a RNA (125 pg): 2-cell (A), 4-cell (B), 8-cell (C), and 16-cell stage (D). (E–L) Phenotypes after microinjection of the DN-p38a RNA (125–250 pg); DN-p38a RNA-injected embryos corresponding to the 2-cell (E and I), 4-cell (F), 8-cell (G and J), and 16-cell (H, K, and L) stage. (F–H) Incomplete cleavages were preferentially located on one side (right) of the first cleavage plane. (I, K, and L) Blastomeres with incomplete cleavages were preferentially located on one side (bottom portion) of the second cleavage plane. (I) Shortened cleavage furrow formation (arrowheads). Incomplete cleavages (arrows, the end of the cleavage furrow ingression) were observed in the intermediate region between the first and second cleavage planes (J). Localization of Myc-tagged DN-p38a (F′; see text). (M) Cross-sections of control embryos at the sphere stage (4 hpf). (N) Magnification of area outlined in M. (O) Cross-sections of the DN-p38a RNA-injected embryos at 4 hpf. (P) Magnification of outlined area in O. (Q) Schematic representation of cleavage formation. Each number denotes the order and sites of the cleavage furrow formation, and the letters in parenthesis correspond to the phenotypes shown in A–P. Asterisks mark each blastomere. Bars, 100 μm.

Impaired symmetric and synchronous cleavage by DN-p38. (A–D) Phenotypes after microinjection of the WT-p38a RNA (125 pg): 2-cell (A), 4-cell (B), 8-cell (C), and 16-cell stage (D). (E–L) Phenotypes after microinjection of the DN-p38a RNA (125–250 pg); DN-p38a RNA-injected embryos corresponding to the 2-cell (E and I), 4-cell (F), 8-cell (G and J), and 16-cell (H, K, and L) stage. (F–H) Incomplete cleavages were preferentially located on one side (right) of the first cleavage plane. (I, K, and L) Blastomeres with incomplete cleavages were preferentially located on one side (bottom portion) of the second cleavage plane. (I) Shortened cleavage furrow formation (arrowheads). Incomplete cleavages (arrows, the end of the cleavage furrow ingression) were observed in the intermediate region between the first and second cleavage planes (J). Localization of Myc-tagged DN-p38a (F′; see text). (M) Cross-sections of control embryos at the sphere stage (4 hpf). (N) Magnification of area outlined in M. (O) Cross-sections of the DN-p38a RNA-injected embryos at 4 hpf. (P) Magnification of outlined area in O. (Q) Schematic representation of cleavage formation. Each number denotes the order and sites of the cleavage furrow formation, and the letters in parenthesis correspond to the phenotypes shown in A–P. Asterisks mark each blastomere. Bars, 100 μm.