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. 2000 Sep 18;150(6):1335–1348. doi: 10.1083/jcb.150.6.1335

Figure 4.

Figure 4

Cell lineage tracing. (A) DN-p38a RNA (125–250 pg) was injected into the yolk cell at 10 mpf, followed by the injection of a cell-tracing dye into one of the blastomeres at the two-cell stage. (B) Tracing dye (rhodamine/biotin-dextran, 2,000 kD) labeled one of the blastomeres of the two-cell stage embryos (red). (C) The labeled blastomere and its daughter cells (red) were located on one side of the blastodisc at the 1,000-cell stage. (D) Cross-section of DN-p38a RNA-injected embryo at the 1,000-cell stage (C, arrow, 100 μm from the animal pole surface). (E–G) Outlined area as in C. The blastomeres with cleavage defects were always located on the labeled side (red). (E–G) Marginal area (demarcated by dotted line) between normally divided (N) and enlarged blastomeres (EN). The blastomeres containing a large amount of cytoplasm and enlarged nucleus were strictly located on either the labeled (E) or the unlabeled side (G), or the labeled side included both enlarged and normal blastomeres (F). The red area in D–G is a superimposition of the tracing dye staining. Bars: (B and C) 110 μm; (D) 80 μm; (E–G) 4 μm.