Figure 5.
Cleavage disruption by DN-p38 is cell autonomous. (A) Cell-tracing dye (rhodamine/biotin-dextran, 2,000 kD) was injected together with DN-p38RNA (125–250 pg) into one of the blastomeres of two-cell embryos. (B) Cross-section of DN/dye-coinjected embryo (A) at 100 μm from the animal pole surface at the 1,000-cell stage. The marginal area, which is the same as that outlined in Fig. 4 D, is shown. Normally divided (N) and enlarged blastomeres (EN) are demarcated by a dotted line. Bar, 4 μm.
