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. 2000 Sep 18;150(6):1335–1348. doi: 10.1083/jcb.150.6.1335

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Effects of UV irradiation on asymmetric p38 activation before the first cleavage formation. Immunostaining of the embryos by either anti-p38 antibody (A and C) or anti–dp-p38 antibody (B and D) after UV irradiation (312 nm) for 10 min from 10 mpf (A and B) or from 25 mpf (C and D) was viewed using a confocal microscope. Asymmetric p38 activation (see Fig. 1B and Fig. C) was totally abolished by UV irradiation at 10 mpf (B, dotted line, outline of the blastodisc). (D) Activation of p38 after UV irradiation at 25 mpf. White lines indicate the site of the first cleavage furrow formation at the two-cell stage. Images were taken as described in the legend for Fig. 1. Bar, 100 μm.