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. 2000 Sep 18;150(6):1335–1348. doi: 10.1083/jcb.150.6.1335

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© 2000 The Rockefeller University Press

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Effects of yolk removal on p38 activation and dharma/bozozok expression. (A) Whole-mount in situ hybridization by dharma RNA probe. Removal of the vegetal yolk hemisphere abolished the expression of dharma (A, left indicated as yolk-depleted; 100%, n = 30) at the sphere stage. dharma transcripts were detected in the dorsal YSL of the control on which no operation was performed (right). (B) Immunostaining of the yolk-depleted embryo at the two-cell stage by anti-p38 antibody to show the distribution of p38, and p38 activation was probed using the anti–dp-p38 antibody (n = 20) (C). Images were taken as described in the legend for Fig. 1. Bars, 100 μm.