Table 2.

Effects of SB203580 on Zebrafish Cleavage

Concentrationof SB203580	n	Percent disruptedcleavage	Percent normalcleavage	Percentdead
μM
10	120	40	60	0
100	138	100	0	0

Fertized embryos were incubated in SB203580 solution, prepared with the indicated concentrations, in E3 medium from the quiescent stage (10–15 mpf) and observed under a microscope at the 20-mpf and at the 16-cell stage to check the cleavage disruption. Embryos were counted at the 16-cell stage. Incubation in DMSO alone (up to 0.5%) did not affect cleavage of normal development.