Figure 2.

Dystrophin Δ71–78 skeletal muscles are morphologically similar to age-matched wild-type muscles. Hematoxylin and eosin staining of quadriceps shows no signs of necrosis or fibrosis in Δ71–78 mice that is apparent in mdx mice (A, 4-mo-old mice; B, 1-yr-old mice). (B) Occasional fibers in 1-yr-old Δ71–78 mice contain central nuclei. (C) Labeling of NMJs reveals no abnormalities in Δ71–78 transgenic muscles in comparison to wild-type muscles. (D) Evans blue dye is excluded from hind limb skeletal muscle fibers in wild-type and Δ71–78, but not mdx mice. (E) Cryosections from diaphragm muscle show Evans blue dye positive fibers only in mdx mice. Scale bars, 50 μm.