Figure 10.

Integrins α_vβ₃ and α_vβ₅ mediate attachment of fetal pancreatic epithelial cells. Established cell monolayers were challenged with anti-α_vβ₃ and anti-α_vβ₅ function blocking antibodies (25 μg/ml) to assess the role of these two integrin receptors in the maintenance of cell monolayer integrity. After 6 h of incubation in the presence of anti-α_vβ₃, mAb cells from the periphery of monolayers started to detach and roll back into three-dimensional clusters (lower middle panel, arrows). Conversely, incubation with the anti-α_vβ₅ mAb resulted in the almost complete detachment of cell monolayers from the matrix (lower right panel). Results shown are representative of four independent experiments. Bar, 150 μm.