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. 2001 Jun 25;153(7):1479–1498. doi: 10.1083/jcb.153.7.1479

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Rate of fluid phase endocytosis, and crown size, in wild-type and p116⁻ cells. (A) Kinetics of accumulation of the fluid phase pinocytic marker FITC-dextran (arbitrary units) within three independent p116⁻ cell lines and the parental line Ax3 (see key). Each value is the mean of duplicate samples. (B) Histogram of the height of crowns in Ax3 and p116⁻ cells (see key). The values, which were taken from confocal Z series of cells stained for actin, were binned in 0.7-μm intervals and are presented as a percent of total crowns counted (64 in Ax3, 58 in p116⁻). Crowns were counted only if they rose almost vertically from the dorsal surface of cells. The bottom of such crowns typically exhibited a spherical, solid disc of actin staining. Above this the crown had the appearance of a ring of fluorescence in each section. Similar results were obtained using cells stained for coronin.