Figure 1.

Distribution of actin tails in infected cells. (A) Phase–contrast microscopy. BS-C-1 cells were infected with VV at 1 PFU/cell and at 12 hpi fixed with methanol and photographed under phase–contrast. The actin tails with dense particles at their tips are visible. (B) Confocal microscopy. RK₁₃ cells were infected with VV as in A and fixed and permeabilized at 9 hpi. Cells were stained with rhodamine-conjugated phalloidin, and a single optical section at the base of the cell is shown. Arrows denote pairs of actin tails that are also illustrated in the vertical optical section shown in C–E. F is a magnification of B, showing two actin tails that are shown in the vertical section in G. Bars, 5 μm.