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Nephronectin is the protein(s) of 70–90 kD recognized by α8β1-AP in kidney extracts. Newborn mouse kidney extracts were immunoprecipitated with antinephronectin antiserum or preimmune serum. The extract, depleted extract, and the immunoprecipitate were analyzed in blots using α8β1-AP. Antinephronectin depleted virtually all α8β1-AP binding activity from the kidney extract, whereas the preimmune serum did not reduce the activity at 70–90 kD responsible for α8β1-AP binding.
