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. 2001 Jul 23;154(2):369–388. doi: 10.1083/jcb.200102028

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Copyright © 2001, The Rockefeller University Press

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Figure 10. — Nonhypercalcemic 1α,25(OH)₂D₃ derivatives induce E-cadherin and inhibit β-catenin–TCF-4 transcriptional activity in SW480-ADH cells, causing β-catenin nuclear export and morphological differentiation. (A) Northern and Western blot analyses of E-cadherin expression in cells treated for 24 h and 48 h, respectively, with various doses of 1α,25(OH)₂D₃, MC903, KH1060, or EB1089. Conditions were as above. (B) β-catenin–TCF-4 transcriptional activity in cells transfected with the TOP-flash and FOP-flash constructs and treated 48 h with 10⁻⁷ M of the indicated compound. Mean values and standard deviation of the mean obtained in duplicates of two independent experiments are shown. (C) Induction of differentiation and nuclear export of β-catenin. Immunofluorescence and confocal laser microscopy analysis of β-catenin expression was done as before. SW480-R and SW620 cells were used as negative control.

Figure 10. — Nonhypercalcemic 1α,25(OH)₂D₃ derivatives induce E-cadherin and inhibit β-catenin–TCF-4 transcriptional activity in SW480-ADH cells, causing β-catenin nuclear export and morphological differentiation. (A) Northern and Western blot analyses of E-cadherin expression in cells treated for 24 h and 48 h, respectively, with various doses of 1α,25(OH)₂D₃, MC903, KH1060, or EB1089. Conditions were as above. (B) β-catenin–TCF-4 transcriptional activity in cells transfected with the TOP-flash and FOP-flash constructs and treated 48 h with 10⁻⁷ M of the indicated compound. Mean values and standard deviation of the mean obtained in duplicates of two independent experiments are shown. (C) Induction of differentiation and nuclear export of β-catenin. Immunofluorescence and confocal laser microscopy analysis of β-catenin expression was done as before. SW480-R and SW620 cells were used as negative control.