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. 1998 Aug 18;95(17):10328–10333. doi: 10.1073/pnas.95.17.10328

Figure 2.

Figure 2

PR-1 protein accumulation in response to treatment with NOS or NO donors. Leaves were treated as indicated and PR-1 protein accumulation after 24 hr was determined by immunoblot analysis. (A) Leaves of Xanthi nc (NN) were infiltrated with SA (0.1 mM) or a NOS (5 units) plus a cofactor/substrate mix (see Materials and Methods). As a control, the cofactor/substrate mix without NOS was used (NOS control). (B) Inhibition of NOS-induced PR-1 accumulation by the NOS inhibitor NMMA (150 μM) or the NO scavenger PTIO (1 mM). The chemicals were added to the injection buffer containing NOS. Also shown are controls with infiltration of NMMA/PTIO delayed for 3 hr after addition of NOS (+ 3 hr), or coinfiltration of SA (in water) with these two chemicals. (C) Induction of PR-1 protein accumulation by NO donors: 3-morpholinosydnonimine-1 (SIN-1) (500 μM), GSNO (500 μM), 1,2,3,4-oxatriazolium-5-amino-3-(3-chloro-2-ethylphenyl)chloride 5024 (500 μM), and SNAP (150 μM).