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. 1998 Aug 18;95(17):10328–10333. doi: 10.1073/pnas.95.17.10328

Figure 3.

Figure 3

Effects of NOS on SA accumulation and PR-1 and PAL gene expression. (A) NOS treatment induces SA accumulation. Levels of total SA (free SA plus conjugated forms) were determined in either untreated leaves or 18 hr after infiltration with NOS or a substrate/cofactor mix lacking NOS (control). In untreated plants, SA was below the detection limit of 0.01 μg/g FW. (B) PR-1 protein accumulation in wild-type (wt) and NahG tobacco in response to treatment with SA or NOS. Treatment of leaves and PR-1 immunoblot analysis were done as described for Fig. 2. (C) Kinetics of PR-1 and PAL mRNA accumulation in response to treatment with NOS. (D) PR-1 and PAL mRNA accumulation in wild-type and NahG tobacco in response to treatment with SA or NOS. Treatment of leaves was as described for Fig. 2. Samples were taken after 8 and 18 hr for PAL and PR-1 mRNA. Ethidium bromide-stained rRNAs are shown as a control for gel loading.