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NO donors induce PAL and PR-1 gene expression in tobacco suspension cells. (A) PR-1 protein and mRNA accumulation after treatment with SA (100 μM), GSNO (200 μM), or SNAP (100 μM). Total RNA was isolated from cells 9 hr posttreatment and subjected to Northern hybridization. Protein samples were taken after 24 hr. (B) Accumulation of PAL mRNA in suspension cells 5 hr posttreatment. Ethidium bromide-stained rRNAs are shown as a control for gel loading.
