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. 2001 Sep 17;154(6):1147–1160. doi: 10.1083/jcb.200101081

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Copyright © 2001, The Rockefeller University Press

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Figure 9. — Turnover of GFP₃-hNup133 on kinetochores. (A and B) GFP₃-hNup133 binding to kinetochores was analyzed by FRAP (A) or FLIP (B) experiments in stable NRK cells. Bleach regions are outlined in the prebleach images. Note the incomplete recovery in FRAP after 8 min (A, 8:15) and the significant fluorescence remaining on kinetochores after cytoplasmic fluorescence was depleted to background in the FLIP (B, 5:13). (C) Plot of mean kinetochore recovery over time of FRAP experiments similar to A. Error bars are standard deviations (n = 10). (D) Plot of mean kinetochore (gray) and cytoplasmic (black) intensity over time with standard deviation (n = 5) of FLIP experiments similar to B. See also Fig. S3 and supplemental methods available at http://www.jcb.org/content/vol154/issue6. Bars, 5 μm.