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. 2001 Sep 17;154(6):1125–1134. doi: 10.1083/jcb.200106011

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Copyright © 2001, The Rockefeller University Press

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Figure 6. — Eg5 distribution in spindles depends on dynactin function. (a) An overlay showing the immunolocalization of endogenous Eg5 (green), tubulin (red), and DNA (blue) in spindles assembled in the presence of p50 dynamitin (0.7 mg/ml), an inhibitor of dynactin. (b) Eg5 alone. (c) An overlay showing the distribution of recombinant labeled Eg5 (green), tubulin, and DNA in spindles assembled in the presence of the dynactin inhibitor. (d) Labeled Eg5 alone. Linescans comparing the distribution of Eg5 and tubulin in spindles assembled in the presence of p50 dynamitin are shown in Fig. 7 c.