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. 2001 Sep 17;154(6):1161–1172. doi: 10.1083/jcb.200104058

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Copyright © 2001, The Rockefeller University Press

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Figure 5. — Induction of HES6 mRNA expression during differentiation of C2C12 myoblasts. The cells were grown to confluence in growth medium then switched to low serum DM. RNA was extracted after 0, 3, and 7 d of differentiation and analyzed by Northern blot assay using the HES6 cDNA. The membrane was then stripped and reprobed with the GAPDH cDNA to monitor for loading variations. Note the induction of HES6 expression during the differentiation of the C2C12 myoblasts into fused myotubes.