Figure 4.

PKCα coimmunoprecipitates specifically with P0 from Schwann cells and L cells expressing full-length P0. Confluent cultures of Schwann cells (SC), L cells expressing full-length P0 (P0wt), and L cells transfected with empty vector (Vect) were lysed in nonionic detergent. 1 ml of each cleared cell lysate was immunoprecipitated with anti-P0 antibody (P0), and equal aliquots of resuspended precipitates were fractionated by SDS-PAGE, transferred to PVDF, and probed with antibody to the PKC isoform indicated. 20 μl of each cell lysate, before immunoprecipitation, was also fractionated by SDS-PAGE, transferred to PVDF, and blotted with the indicated antibody (lanes labeled none). Numbers to the left indicate the migration of prestained standards. Bands due to PKC and Ig heavy chain are indicated at the right.