Figure 6.

Dominant negative Fyn inhibits disassembly of hemidesmosomes and suppresses cell migration. The indicated 804G clones were grown until confluent and then serum starved. After wounding, the monolayers were treated with EGF (50 ng/ml) for 5 or 10 h or left untreated for 5 h, and then either stained with Crystal violet or subjected to immunofluorescent staining with affinity purified anti–BPAG-2 antibodies. Cell migration results were quantitated as described in the Materials and methods section and plotted graphically. The graph shows the mean and SD of values from a representative experiment performed in triplicate. Results similar to those shown here were obtained with all three clones expressing dominant negative Fyn and the two clones expressing dominant negative Src. Bar, 250 μm.