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. 2001 Oct 29;155(3):355–368. doi: 10.1083/jcb.200106123

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Copyright © 2001, The Rockefeller University Press

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Figure 5. — Degradation of KHN _t and CPY* _HA but not Ste6–166p requires Golgi-to-ER transport. Pulse–chase analysis was performed on wild-type and sec21–1 strains expressing (A) KHN_t, (B) CPY*_HA, and (C) Ste6–166p as described in the legend to Fig. 2 except that strains were grown to log phase at 22°C and pulse-labeled immediately after a shift to 33°C. Incubation at 33°C was continued for the cold chase (times as indicated). Gels were visualized by autoradiography (left) and quantified by PhosphorImager analysis (right). In C, the gel images were from PhosphorImager scans.