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. 2001 Oct 29;155(3):355–368. doi: 10.1083/jcb.200106123

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Copyright © 2001, The Rockefeller University Press

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Figure 6. — per17–1 is a mutant specific to the retrieval pathway, which blocks the transport of misfolded proteins but not properly folded proteins. (A) The turnover of KHN_t, CPY*_HA, Ste6–166p, and Sec61–2p in wild-type and per17–1 cells were measured by metabolic pulse–chase analysis as described in the legend to Fig. 2. Experiments were performed at 30°C except for strains expressing Sec61–2p. Strains expressing Sec61–2p were grown to log phase at 30°C, shifted to 37°C for 30 min, and continued for the pulse–chase. (B) Autoradiograms generated from gels of the KHN_t time course shown in part A are shown at the top. The positions of the p1 (ER) and p2 (Golgi-modified) forms are indicated. Endogenous CPY and Gas1p were immunoprecipitated in parallel from aliquots of lysates prepared from the KHN_t time course. The proteins were separated by gel electrophoresis and visualized by autoradiography (P1, ER proCPY; P2, Golgi proCPY; mCPY, mature CPY; ER Gas1p, ER form of Gas1p; mGas1, mature Golgi-modified Gas1p). (C) Wild-type and per17–1 cells were pulse labeled for 10 min and chased for times indicated. CPS and ALP were immunoprecipitated and analyzed by gel electrophoresis followed by autoradiography. The pro (proCPS and proALP) and mature (mCPS and mALP) forms of each protein are indicated.

Figure 6. — per17–1 is a mutant specific to the retrieval pathway, which blocks the transport of misfolded proteins but not properly folded proteins. (A) The turnover of KHN_t, CPY*_HA, Ste6–166p, and Sec61–2p in wild-type and per17–1 cells were measured by metabolic pulse–chase analysis as described in the legend to Fig. 2. Experiments were performed at 30°C except for strains expressing Sec61–2p. Strains expressing Sec61–2p were grown to log phase at 30°C, shifted to 37°C for 30 min, and continued for the pulse–chase. (B) Autoradiograms generated from gels of the KHN_t time course shown in part A are shown at the top. The positions of the p1 (ER) and p2 (Golgi-modified) forms are indicated. Endogenous CPY and Gas1p were immunoprecipitated in parallel from aliquots of lysates prepared from the KHN_t time course. The proteins were separated by gel electrophoresis and visualized by autoradiography (P1, ER proCPY; P2, Golgi proCPY; mCPY, mature CPY; ER Gas1p, ER form of Gas1p; mGas1, mature Golgi-modified Gas1p). (C) Wild-type and per17–1 cells were pulse labeled for 10 min and chased for times indicated. CPS and ALP were immunoprecipitated and analyzed by gel electrophoresis followed by autoradiography. The pro (proCPS and proALP) and mature (mCPS and mALP) forms of each protein are indicated.