Table I. Strains used in this study.
| Strain | Genotype | Source |
|---|---|---|
| W303a | MAT a, leu2-3,112, his3-11, trp1-1, ura3-1, can1-100, ade2-1 | P. Waltera |
| SMY248 | MAT a, W303, pSM70 | This study |
| SMY249 | MAT a, cue1::TRP1, pSM70, W303 background | This study |
| SMY 250 | MAT a, der1::KANMX, pSM70, W303 background | This study |
| SMY251 | MAT a, der3::KANMX, pSM70, W303 background | This study |
| SMY252 | MAT a, per17-1, pSM70, W303 background | This study |
| SMY254 | MATα, pep4::HIS3, pSM70, W303 background | This study |
| SMY258 | MAT a, sec12-4, pSM70, W303 background | This study |
| SMY259 | MAT a, sec18-1, pSM70, W303 background | This study |
| RSY255 | MATα, leu2-3,112, ura3-52 | R. Schekmanb |
| RSY277 | MATα, sec21-1, ura3-52 | R. Schekman |
| SMY359 | MATα, RSY255, pSM70 | This study |
| SMY360 | MATα, RSY255, pDN431 | This study |
| SMY361 | MATα, RSY277, pSM70 | This study |
| SMY362 | MATα, RSY277, pDN431 | This study |
| SMY390 | MAT a, RSY255, pSM1083 | This study |
| SMY391 | MAT a, RSY277, pSM1083 | This study |
| SMY301 | MAT a, W303, pSM31 | This study |
| SMY302 | MAT a, sec12-4, pSM31, W303 background | This study |
| SMY303 | MAT a, sec18-1, pSM31, W303 background | This study |
| SMY312 | MAT a, cue1::TRP1, pSM31, W303 background | This study |
| SY114 | MATα, leu2-3, ura3-52, his3-Δ200, lys2-801, trp1-Δ90, suc2-Δ9 | S. Sandersc |
| SY655 | MATα, pmt1::URA3, SY114 background | S. Sanders |
| SY656 | MATα, pmt2::LEU2, SY114 background | S. Sanders |
| SY657 | MATα, pmt3::HIS3, SY114 background | S. Sanders |
| SY415 | MATα, pmt4::TRP1, SY114 background | S. Sanders |
| SY658 | MATα, pmt5::URA3, SY114 background | S.Sanders |
| SY659 | MATα, pmt6::URA3, SY114 background | S. Sanders |
| SMY319 | MATα, pmt1::URA3, pSM56, W303 background | This study |
| SMY347 | MATα, pmt2::LEU2, pSM56, W303 background | This study |
| SMY313 | MATα, SY656, pSM31 | This study |
| SMY320 | MAT a, SMY237, pSM56, W303 background | This study |
| SMY326 | MAT a, SMY239, pSM31, W303 background | This study |
| SMY348 | MAT a, W303, pSM1346, W303 background | This study |
| SMY349 | MAT a, per17-1, SM1346, W303 background | This study |
| SMY225 | MAT a, W303, pSM1083, W303 background | This study |
| SMY226 | MAT a, cue1::TRP1, pSM1083, W303 background | This study |
| SMY227 | MAT a, sec12-4, pSM1083, W303 background | This study |
| SMY228 | MAT a, sec18-1, pSM1083, W303 background | This study |
| WKY4 | MAT a, sec12-4, pDN431, W303 background | This study |
| WKY20 | MAT a, per17-1, pDN436, W303 background | This study |
| WKY21 | MAT a, per17-1, pDN1002, W303 background | This study |
| WKY23 | MAT a, sec12-4, pDN1002, W303 background | This study |
| WKY25 | MAT a, sec18-1, pDN1002, W303 background | This study |
| WKY108 | MAT a, per17-1, pSM1346, W303 background | This study |
| WKY110 | MAT a, W303, pSM1346, W303 background | This study |
| WKY135 | MAT a, sec18-1, pDN436, W303 background | This study |
| SMY340 | MAT a, W303, pSM70, pSM72 | This study |
| SMY342 | MAT a, per17-1, pSM70, pSM72, W303 background | This study |
| SMY383 | MAT a, der1::KANMX, pSM70, pSM72, W303 background | This study |
| SMY384 | MAT a, der3:KANMX, pSM70, pSM72, W303 background | This study |
| WKY213 | MAT a, W303, pDN436 | This study |
| SMY385 | MAT a, W303, pES69 | This study |
| SMY387 | MAT a, per17-1, pES69, W303 background | This study |
a
University of California, San Francisco, CA.
b
University of California, Berkeley, CA.
c
Massachusetts Institute of Technology, Cambridge, MA.