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. 2001 Nov 26;155(5):763–774. doi: 10.1083/jcb.200105029

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Copyright © 2001, The Rockefeller University Press

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Figure 9. — Association of Ipl1 and Sli15 with centromeric DNA. Extracts prepared from formaldehyde-fixed yeast cells (CCY776-5D) that did not (untagged) or did express HA–Ipl1 (pCC1128) or Sli15–Myc (pCC1193) were prepared. These extracts were left untreated (WCE), mock-precipitated without antibodies (No Ab), or immunoprecipitated with anti-HA (α HA) or anti-Myc (α Myc) antibodies. PCR was performed to amplify DNA fragments from WCE or precipitated samples, using primers that flank CEN3 or CEN16, or primers from a region located ∼1 kb from CEN16.