Table I. Plasmids and yeast strains used in this study.
| Name | Relevant features | Source |
|---|---|---|
| pCC510 | URA3, 2m, GLC7 | Francisco et al., 1994 |
| pCC669 | lacIq, GST-IPL1 under tac promoter control (in pGEX-3X) | This study |
| pCC929 | TRP1, CYH2, 2μ, GAL4BD-IPL1 under ADH1 promoter (in pAS2) | This study |
| pCC1007 | LEU2, 2μ, GAL4AD-SLI15611-698 under ADH1 promoter (in pGAD-C2) | This study |
| pCC1053 | LEU2, 2μ, GAL4AD-IPL1 under ADH1 promoter (in pGAD-C2) | This study |
| pCC1060 | URA3, CEN, GFPS65T-SLI15 under ACT1 promoter (in pRB2138) | Kim et al., 1999 |
| pCC1061 | URA3, leu2-d, 2μ, GST-SLI15 under GAL1/10 promoter (in pEG(KT)) | Kim et al., 1999 |
| pCC1062 | lacIq, GST-SLI15 under tac promoter control (in pGEX-2T) | Kim et al., 1999 |
| pCC1128 | LEU2, 2μ, HA-IPL1 | Kim et al., 1999 |
| pCC1173 | LEU2, 2μ, SLI15-Myc | Kim et al., 1999 |
| pCC1193 | URA3, 2μ, SLI15-Myc | Kim et al., 1999 |
| pCC1212 | TRP1, 2μ, GAL4BD-SLI15 under ADH1 promoter (in pGBD-C2) | This study |
| pCC1271 | lacIq, GST-IPL1D245N under tac promoter control (in pGEX-3X) | This study |
| pCC1301 | LEU2, 2μ, SLI15-HA | This study |
| pCC1309 | URA3, 2μ, GAL4BD-SLI15 under ADH1 promoter (in pGBDU-C2) | This study |
| pCC1428 | LEU2, 2μ, GAL4AD-DUO1110-247 under ADH1 promoter (in pGAD-C3) | This study |
| pCC1469 | lacI q, GST-SLI151-229 under tac promoter control (in pGEX-2T) | This study |
| pCC1470 | lacI q, GST-SLI15227-559 under tac promoter control (in pGEX-2T) | This study |
| pCC1471 | lacI q, GST-SLI15558-698 under tac promoter control (in pGEX-1) | This study |
| pCC1533 | URA3, CEN, GFPS65T-SLI151-229 under ACT1 promoter (in pRB2138) | This study |
| pCC1534 | URA3, CEN, GFPS65T-SLI15227-559 under ACT1 promoter (in pRB2138) | This study |
| pCC1560 | TRP1, 2μ, GAL4BD-SLI151-229 under ADH1 promoter (in pGBD-C1) | This study |
| pCC1561 | TRP1, 2μ, GAL4BD-SLI15227-559 under ADH1 promoter (in pGBD-C1) | This study |
| pCC1562 | TRP1, 2μ, GAL4BD-SLI15558-698 under ADH1 promoter (in pGBD-C1) | This study |
| pCC1563 | URA3, leu2-d, 2μ, GST-SLI151-229 under GAL1/10 promoter (in pEG(KT)) | This study |
| pCC1564 | URA3, leu2-d, 2μ, GST-SLI15227-559 under GAL1/10 promoter (in pEG(KT)) | This study |
| pCC1565 | URA3, leu2-d, 2μ, GST-SLI15558-698 under GAL1/10 promoter (in pEG(KT)) | This study |
| pCC1566 | URA3, CEN, GFPS65T-SLI15558-698 under ACT1 promoter (in pRB2138) | This study |
| pCC1575 | LEU2, 2μ, DAM1 | This study |
| pCC1584 | TRP1, CEN, GFPS65T-IPL1 under ACT1 promoter (in pRS314) | This study |
| pDD475 | URA3, leu2-d, 2μ, GST-DUO1 under GAL1/10 promoter (in pEG(KT)) | Hofmann et al., 1998 |
| pDD884 | lacIq, His6-DAM1 under T7 promoter (in pRSETa) | Cheeseman et al., 2001 |
| pDD1017 | URA3, leu2-d, 2μ, GST-DAM1 under GAL1/10 promoter (in pEG(KT)) | This study |
| pDD1018 | LEU2, 2μ, GAL4AD-DAM1 under ADH1 promoter (in pACT2) | This study |
| pDD1019 | lacIq, His6-GST-DAM1175-343 under T7 promoter (in pGAT2) | This study |
| pDD1020 | LEU2, 2μ, GAL4AD-DUO1 under ADH1 promoter (in pACT2) | This study |
| pEG(KT) | URA3, leu2-d, 2μ, GST under GAL1/10 promoter | Mitchell et al., 1993 |
| pGEX-2T | lacIq, GST under tac promoter | Smith and Johnson, 1988 |
| pRS316 | URA3, CEN | Sikorski and Hieter, 1989 |
| CCY766-5D | α lys2-801 his3-Δ200 ura3-52 leu2-3,112 trp1-1 | This study |
| CCY766-9D | a lys2-801 his3-Δ200 ura3-52 leu2-3,112 trp1-1 | Kim et al., 1999 |
| CCY915-13C | a lys2-801 his3-Δ200 ura3-52 leu2-3,112 trp1-1 ipl1-2 | Kim et al., 1999 |
| CCY1124-4B | α lys2-801 his3-Δ200 ura3-52 leu2-3,112 sli15-3 | This study |
| DBY1830 | a /α ade2/1 lys2-801/1 his3-Δ200/his3-Δ200 ura3-52/ura3-52 leu2-3,112/leu2-3,112 trp1-1/trp1-1 | Kim et al., 1999 |
| DDY904 | α lys2-801 his3-Δ200 ura3-52 leu2-3,112 | D. Drubin |
| DDY1906 | α lys2-801 his3-Δ200 ura3-52 leu2-3,112 dam1-9::KanMX | Cheeseman et al., 2001 |
| DDY1910 | α lys2-801 his3-Δ200 ura3-52 leu2-3,112 dam1-11::KanMX | Cheeseman et al., 2001 |
| DDY1914 | α lys2-801 his3-Δ200 ura3-52 leu2-3,112 dam1-1::KanMX | Cheeseman et al., 2001 |
| DDY1525 | α lys2-801 his3-Δ200 ura3-52 leu2-3,112 duo1-2::LEU2 | Hofmann et al., 1998 |
| PJ69-4A | a trp1-901 leu2-3,112 ura3-52 his3-Δ200 gal4Δ gal80Δ LYS2::GAL1→HIS3 GAL2→AΔE2 met2::GAL7-lacZ | James et al., 1996 |
| TD4 | a ura3-52 leu2-3,112 trp1-289 his4-519 | Kim et al., 1999 |
| Y190 | a gal4 gal80 his3 trp1-901 ade2-101 ura3-52 leu2-3,112 URA3::GAL→lacZ LYS2::GAL→HIS3 cyhr | Harper et al., 1993 |
All plasmids contain the ampicillin-resistance marker for selection in E. coli.