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Nuclear translocation of CD44ICD (A) COS-7 cells were transfected with HA-tagged CD44ICD (left) or Myr-CD44ICD (right) expression plasmids. After 24 h, the cells were stained with anti-HA mAb. (B) U251MG cells were treated as described in the legend to Fig. 2, A or B. Membrane/cytosol and nuclear fractions were analyzed by Western blotting with anti-CD44cyto Ab, anti-MEK2 Ab (cytosolic marker), and anti-Histone H1 (nuclear marker). Bars, 20 μm.
