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. 2001 Nov 26;155(5):775–786. doi: 10.1083/jcb.200105044

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Copyright © 2001, The Rockefeller University Press

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Figure 7. — Raver1 shuttles between nuclear and cytoplasmic compartments in heterokaryons. HeLa cells were transiently transfected with FLAG-raver1, mixed and subsequently fused with mouse C2C12 cells, and incubated for another 4 h in the presence of cycloheximide. The coculture was fixed and stained using FLAG antibody. (A and D) Phase–contrast images of two examples, showing larger and smaller (arrowheads) nuclei within one common cytoplasm. (B, E, and insets) Identification by DAPI staining of the smaller nuclei (arrowheads) as of murine origin containing multiple DNA dots and the larger nuclei as human without prominent dots. (C and F) FLAG-raver1 is found within murine nuclei in fusion products with the raver1-transfected HeLa cells (arrowheads) but not in the corresponding nuclei in the untransfected unfused C2C12 cells (B and E, arrows). Bars, 10 μm.