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. 2001 Nov 26;155(5):775–786. doi: 10.1083/jcb.200105044

Table II. Constructs generated for this study.

Construct Insert Vector
His-FLAG-VT His/FLAG-tagged murine vinculin tail (vt), amino acids 858–1066 pQE-30
His-FLAG-MVT His/FLAG-tagged murine metavinculin tail (mvt), amino acids 858–1134 pQE-30
MBP-raver1/prey MBP-tagged murine raver1 fragment, amino acids 1–625 pMalc2
FLAG-VT FLAG-tagged vt, amino acids 858–1066 pcDNA3
FLAG-MVT FLAG-tagged mvt, amino acids 858–1134 pcDNA3
FLAG-raver1 FLAG-tagged raver1, amino acids 1–748 pcDNA3
FLAG-ZBP1 FLAG-tagged ZBP1, amino acids 1–576 pcDNA3
4A6-raver1 Birch profilin epitope-tagged raver1, amino acids 1–748 pcDNA3
Gal4BD-VT Gal4(BD-domain)-tagged vt, amino acids 858–1066 pGBT-9
Gal4BD-MVT Gal4(BD-domain)-tagged mvt, amino acids 858–1134 pGBT-9
Gal4AD-VT Gal4(AD-domain)-tagged vt, amino acids 858–1066 pGADT7
Gal4AD-VTΔC1 Gal4(AD-domain)-tagged vt, amino acids 858–1012 pGADT7
Gal4AD-VTΔC2 Gal4(AD-domain)-tagged vt, amino acids 858–975 pGADT7
Gal4AD-VTΔC3 Gal4(AD-domain)-tagged vt, amino acids 858–942 pGADT7
Gal4AD-MVT Gal4(AD-domain)-tagged mvt, amino acids 858–1134 pGADT7
Gal4AD-MVTΔC1 Gal4(AD-domain)-tagged vt, amino acids 858–1080 pGADT7
Gal4AD-MVTΔC2 Gal4(AD-domain)-tagged vt, amino acids 858–1043 pGADT7
Gal4AD-MVTΔC3 Gal4(AD-domain)-tagged vt, amino acids 858–1010 pGADT7
Gal4BD-raver1 Gal4(BD-domain)-tagged raver1, complete pGADT7
Gal4BD-raver1NΔC Gal4(BD-domain)-tagged raver1, amino acids 1–441 pGADT7
Gal4BD-raver1CΔN Gal4(BD-domain)-tagged raver1, amino acids 442–748 pGADT7
Gal4AD-PTB1 Gal4(AD-domain)-tagged PTB1, amino acids 1–557 pGADT7