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. 2001 Dec 10;155(6):1017–1028. doi: 10.1083/jcb.200106023

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Copyright © 2001, The Rockefeller University Press

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Figure 6. — Repression of Smad2 phosphorylation by I-Smads, their deletion mutants, and chimeras. COS7 cells were transfected with the indicated plasmids, and phosphorylation of Smad2 was determined by FLAG immunoprecipitation of Smad2, followed by phosphoserine immunoblotting (top). Levels of expression for each protein were determined by immunoblotting using FLAG, HA, or Myc antibodies (lower). + and ++ are 0.1 and 0.3 μg of DNA, respectively, transfected in COS7 cells.