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. 2001 Dec 10;155(6):1017–1028. doi: 10.1083/jcb.200106023

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Copyright © 2001, The Rockefeller University Press

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Figure 7. — Physical interaction of Smads with TGF-β receptors. (A) Affinity cross-linking using ¹²⁵I–TGF-β1 followed by immunoprecipitation by anti-FLAG antibody was performed using COS7 cells transfected with the indicated plasmids (top). Levels of expression of I-Smads and their mutants and chimeras were examined by immunoblotting using anti-FLAG antibody (bottom). (B) Interaction of I-Smads with TβR-I(TD) was examined by FLAG immunoprecipitation of I-Smads, followed by HA-immunoblotting of TβR-I(TD) using transfected COS7 cells (top). The Smad6 and Smad7 chimeras shown in Fig. 3 A were used. 6(32), S6(31/32)S7; 6(91), S6(171/91)S7; 6(158), S6(225/158); 6(211), S6(279/211)S7. Note that HA-tagged TβR-I(TD) was comigrated with IgG. Lower panels demonstrate the levels of expression of I-Smads (middle) and TβR-I(TD) (bottom).

Figure 7. — Physical interaction of Smads with TGF-β receptors. (A) Affinity cross-linking using ¹²⁵I–TGF-β1 followed by immunoprecipitation by anti-FLAG antibody was performed using COS7 cells transfected with the indicated plasmids (top). Levels of expression of I-Smads and their mutants and chimeras were examined by immunoblotting using anti-FLAG antibody (bottom). (B) Interaction of I-Smads with TβR-I(TD) was examined by FLAG immunoprecipitation of I-Smads, followed by HA-immunoblotting of TβR-I(TD) using transfected COS7 cells (top). The Smad6 and Smad7 chimeras shown in Fig. 3 A were used. 6(32), S6(31/32)S7; 6(91), S6(171/91)S7; 6(158), S6(225/158); 6(211), S6(279/211)S7. Note that HA-tagged TβR-I(TD) was comigrated with IgG. Lower panels demonstrate the levels of expression of I-Smads (middle) and TβR-I(TD) (bottom).