Figure 1.

Organization of and the complete nucleotide sequence of LE-ACS7. (A) The graph shows the organization of the λ-EMBL3 clone pPLT1. Three exons of LE-ACS7 are represented by dotted boxes, and two introns are represented by lines connecting the three exons. (B) Complete nucleotide sequence of the LE-ACS7 gene. The coding sequence is capitalized and highlighted, and introns and untranslated regions are indicated in lowercase. Putative tata-box, cap site, and polyadenylation sites are underlined in bold. A long, imperfect, inverted repeat is underlined. Regions corresponding to regions C, D, and E of the tomato polygalacturonase promoter (31) are shaded, shaded and underlined, and shaded and double underlined, respectively. The amino acid sequence is presented above the DNA sequence and numbered separately. The dodecapeptide that is part of the reactive center of ACC synthase is underlined. The star identifies the stop codon. The seven invariant residues that bind the pyridoxal phosphate coenzyme in various aspartate aminotransferases are conserved across all aspartate aminotransferases, and ACC synthase are in bold. The region of the p4ROM2 probe is delimited by two inverted triangles (nt 2,632–3,456).