Figure 4.

Expression of ACC synthase genes LE-ACS2, LE-ACS3, and LE-ACS7 in leaves and roots of flooded plants. RNA was extracted at various times after flooding, and 100 μg for each time point and gene was analyzed by RPA assay. A section of an ethidium bromide-stained agarose gel with ribosomal RNA bands is depicted under F to show that even amounts of RNA were used for the assays. The times of flooding (h) are given above the figures. (A–C) Expression of LE-ACS2, LE-ACS3, and LE-ACS7, respectively, in leaves and (D–F) in roots.