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. 2001 Dec 10;155(6):923–936. doi: 10.1083/jcb.200108142

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Copyright © 2001, The Rockefeller University Press

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Figure 6. — Semiquantitative analysis of the labeling distribution in the nucleolus and the nucleoplasm after in situ hybridization with the 18S probe. Pictures of cells grown at 34°C and prepared as in Fig. 5 were taken randomly; on each picture, the labeling density of the nucleolus (d_No) and of the nucleoplasm (d_Np) was evaluated as described in Materials and methods, and the ratio d_No/d_Np was calculated. The graph shows the mean values for each strain. A one to one comparison of the mean value of the wild-type strain (FY86) with the others was performed (* P<0.05; ** P<0.01; *** P<0.001). Two independent in situ hybridization experiments were necessary to examine all the strains. The results obtained for the wild-type cells in these two experiments are shown (WT #1 and WT #2).