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. 2005 Dec 16;62(Pt 1):70–73. doi: 10.1107/S1744309105041473

Figure 1.

Figure 1

HLA-G refolding and functional testing. (a) SDS–PAGE analysis of refolded HLA-­G–RIIPRHLQL complex following anion-exchange and size-exclusion chromatography. (b) ELISA of refolded HLA-G. Two antibodies specific for human MHC molecules were used in a sandwich ELISA with HLA-G (filled squares) and HLA-B8 (filled triangles) as positive controls and LC13 (filled circles) as a negative control. Open symbols indicate the control experiment without primary antibody. Data represents the mean of three experiments.