Table III.
NaCl dependence of M3–XCL1 binding kinetics
| NaCl (M) | KD(eq) (Req) (nM) | KD(kd/ka) (nM) | kaapp (M−1s−1) ×107 | kdapp (s−1) ×10−2 | χ2 avga |
|---|---|---|---|---|---|
| 0.2 | − | 0.9 (±0.2) | 6 (±1) | 5.6 (±0.2) | 21.61 |
| 0.25 | 2.6 (±0.4) | 1.8 (±0.4) | 5 (±2) | 9 (±3) | 2.15 |
| 0.3 | 4.6 (±0.5) | 3 (±0.5) | 3 (±1) | 9 (±2) | 0.49 |
| 0.35 | 6.9 (±0.8) | 4.6 (±0.6) | 2.2 (±0.7) | 10 (±2) | 0.35 |
| 0.4 | 9 (±0.9) | 7 (±1) | 1.6 (±0.4) | 10 (±2) | 0.4 |
| 0.5 | 11 (±1) | 9 (±1) | 0.9 (±0.2) | 9 (±1) | 0.3 |
| 0.6 | 16.2 (±0.8) | 12 (±1) | 0.33 (±0.04) | 3.9 (±0.6) | 0.49 |
| 0.7 | 18.8 (±0.9) | 17 (±2) | 0.24 (±0.02) | 4.2 (±0.5) | 0.69 |
| 1 | 21 (±2) | 17 (±2) | 0.19 (±0.03) | 3.1 (±0.3) | 0.56 |
| 1.25 | 28 (±2) | 19 (±0.8) | 0.13 (±0.01) | 2.5 (±2) | 0.37 |
| 1.5 | 26 (±6) | 22 (±2) | 0.083 (±0.003) | 1.9 (±0.1) | 0.52 |
a
Average χ2 for global kinetic fits at three ligand densities measured in triplicate.