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. 2007 Dec 24;204(13):3271–3283. doi: 10.1084/jem.20071155

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Copyright © 2007, The Rockefeller University Press

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Figure 5. — RNA-FISH to assess the transcriptional activity of the H chain alleles and their V_H gene usage. Detection with an Iμ probe indicated whether one or both of the IgH loci was actively transcribing, and detection with a J558 or, separately, a 7183 probe revealed the V_H gene usage of V_HDJ_H-rearranged alleles. Cells from normal mice (NM) and L^−/− mice were analyzed in parallel. (A) Representative signal combinations detected for Iμ (red) and J558 (green) transcripts in sorted B220⁺ CD25⁺ L^−/− bone marrow cells. Bar, 5 μm. (B–D) Comparison of signal ratios in sorted B220⁺ CD25⁺ bone marrow cells from normal and L^−/− mice stained for (B) Iμ, (C) Iμ and J558, and (D) Iμ and 7183 transcripts. SD was calculated from four separate experiments, whereas representative plots (C and D) were repeated at least once with similar results.