Figure 2.
O2 cRSs are found at multiple locations within mouse VH gene segments. RIC12 was computed for all 28-bp segments embedded in mouse VH gene segments. RIC12 scores of potential cRSs (RIC 12 > −55) are plotted against the segment's nucleotide position (IMGT numbering). Open circles (○) indicate potential 12-cRSs and filled circles (•) indicate those segments with RIC12> −48.2, the lowest RIC12 for which we have detected extrachromosomal recombination (18). Locations of CDR1, -2, and -3 are shown by the shaded areas of the graphs. Roman numerals indicate clusters of cRSs that are conserved across VH gene families. Site I spans amino acid residues 18–22 (nt 54–63); site II spans amino acid residues 34–42 (nt 100–126); site III spans residues 50–62 (nt 148–184); site IV spans residues 64–69 (nt 190–205); and site V is amino acid residue 105 (nt 313).