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. 2007 Dec 24;204(13):3235–3245. doi: 10.1084/jem.20071239

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Copyright © 2007, The Rockefeller University Press

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Figure 6. — An intact TTSS is required for P. aeruginosa-mediated caspase-1 activation. LPS-stimulated WT macrophages were infected with the P. aeruginosa strain PA103ΔUT, PA103MutN, PA103Mut1, PA103ΔpilA, PA103ΔpopB, or PA103ΔpopD at a MOI of 20 bacteria per macrophage. At the indicated time (A and B) or 3 h (D and E) after infection, culture supernatants were collected and cytotoxicity was measured by LDH release and expressed as a percentage of LDH release by Triton X-100 detergent. IL-1β release into the culture supernatant was measured by ELISA. Determinations were performed in triplicate and expressed as the mean ± the SD. Results are representative of two (A and B) or three (D and E) separate experiments. (C) Lysates from LPS-stimulated WT macrophages that were infected with the P. aeruginosa strain PA103ΔUT, PA103MutN, PA103Mut1, or PA103ΔpilA for 3 h were immunoblotted with antibodies against the p10 subunit of caspase-1.