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. 2007 Dec 24;204(13):3235–3245. doi: 10.1084/jem.20071239

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Copyright © 2007, The Rockefeller University Press

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Figure 7. — IPAF-dependent activation of caspase-1 by P. aeruginosa does not require flagellin. (A) LPS-stimulated macrophages from WT or IPAF-deficient mice were infected with P. aeruginosa strain PAK or the flagellin-deficient PAKΔfliC at a MOI of 20 bacteria per macrophage. 1 h after infection, culture supernatants were collected, and IL-1β release into culture supernatants was measured by ELISA. Determinations were performed in triplicate and expressed as the mean ± the SD. Results are representative of three separate experiments. (B and C) LPS-stimulated macrophages from WT or IPAF-deficient mice were infected with P. aeruginosa strain PAKΔfliC at a MOI of 20 bacteria per macrophage. (B) At the indicated times after infection, culture supernatants were collected and IL-1β release into culture supernatants was measured by ELISA. (C) Lysates were immunoblotted with antibodies against the p10 subunit of caspase-1. Determinations were performed in triplicate and expressed as the mean ± the SD. Results are representative of two separate experiments.